Exploring Antibiotic Resistance Pattern of Escherichia coli, Salmonella spp., and Staphylococcus spp. Isolated from Eggs in Rajshahi
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Chicken eggs are a major component of people’s diets, with an average yearly consumption of approximately 103 eggs per person in Bangladesh. Eggs act as an important carrier of food-borne pathogen worldwide. The study was conducted to identify the prevalence and antibiotic resistance pattern of E. coli, Salmonella spp., and Staphylococcus spp., in eggs isolated from farms and different markets of Rajshahi, Bangladesh. A total of 60 eggs were collected randomly between April to December 2019. The isolation and identification of bacterial pathogen was done in accordance with standard procedures. The bacterial isolates were subjected to antibiotic susceptibility testing against seven commonly used antibiotics using Kirby-Bauer disk diffusion method. An overall prevalence of E. coli, Salmonella spp., and Staphylococcus spp. were found to be 35.0%, 28.33%, and 23.33%, respectively. E. coli were found highly resistant to penicillin (100%), tetracycline (80.95%), ampicillin (100%), and erythromycin (85.71%) and were sensitive to amoxicillin (71.42%), ciprofloxacin (85.71%), and gentamicin (95.23%). Salmonella spp. was highly resistant to penicillin (100%), erythromycin (82.35%) and tetracycline (82.35%), and was sensitive to gentamicin (94.11%), amoxicillin (76.47%) and ciprofloxacin (70.58%). Staphylococcus spp. was resistant to penicillin (100%), erythromycin (78.57%), tetracycline (85.71%), amoxicillin (100%), and ampicillin (100%) but sensitive to ciprofloxacin (85.71%), and gentamicin (92.85%). The higher prevalence of multidrug-resistant (MDR) bacteria can easily enter the food chain, which poses a public health threat.
References
-
FAO, “the state of food security and nutrition in the world 2018: building climate resilience for food security and nutrition”, 2018.
Google Scholar
1
-
D. Stępień-Pyśniak, “Occurrence of Gram-negative bacteria in hen’s eggs depending on their source and storage conditions,” Pol. J. Vet. Sci., Vol. 13, pp. 507-513, Jan. 2010.
Google Scholar
2
-
M. J. Hossain, M. S. Islam, M. A. Sobur, S. B. Zaman, A. Nahar, M. Rahman, M. T. Rahman, “Exploring Poultry Farm Environment for Antibiotic Resistant Escherichia coli, Salmonella spp., and Staphylococcus spp. Having Public Health Significance,” J Bangladesh Agril Univ, Vol. 18, pp. 615–622, Sep 2020.
Google Scholar
3
-
M. H. Haque, S. Sarker, M. S. Islam, et al., “Sustainable Antibiotic-Free Broiler Meat Production: Current Trends, Challenges, and Possibilities in a Developing Country Perspective,” Biology (Basel), Vol. 9, pp. 411, Nov 2020.
Google Scholar
4
-
S. Schwarz, A. Loeffler, K. Kadlec, “Bacterial resistance to antimicrobial agents and its impact on veterinary and human medicine,” Vet Dermatol., Vol. 28, pp. 82-e19, Feb 2017.
Google Scholar
5
-
G. M. Boovaragamoorthy, M. Anbazhagan, P. Piruthiviraj, A. Pugazhendhi, S. S. Kumar, et al., “Clinically important microbial diversity and its antibiotic resistance pattern towards various drugs,” J Infect Public Health, Vol. 12, pp. 783-788, Dec 2019.
Google Scholar
6
-
K Clifford, D. Darash, C. P. da Costa, H. Meyer, M. T. Islam, et al., “The threat of antimicrobial resistance opportunities for a technology-integrated One Health approach,” Bulletin of the World Health Organization, Vol. 96, pp. 662-664, 2018.
Google Scholar
7
-
B. Aslam, W. Wang, M. I. Arshad, et al., “Antibiotic resistance: a rundown of a global crisis,” Infect Drug Resist., Vol. 11, pp. 1645-1658, Oct 2018.
Google Scholar
8
-
F. Prestinaci, P. Pezzotti A. Pantosti, “Antimicrobial resistance: a global multifaceted phenomenon,” Pathog Glob Health, Vol.109, pp. 309-318, Sep 2015.
Google Scholar
9
-
B. Li, and T. J. Webster, “Bacteria antibiotic resistance: New challenges and opportunities for implant-associated orthopedic infections,” J Orthop Res., Vol. 36, pp. 22-32, Jan 2018.
Google Scholar
10
-
S. Thanner, D. Drissner, and F. Walsh, “Antimicrobial Resistance in Agriculture,” mBio., Vol. 7, pp. e02227-15, Apr 2016.
Google Scholar
11
-
V. V. Saharan, P. Verma, A.P. Singh, “Escherichia coli, Salmonella spp., and Staphylococcus aureus susceptibility to antimicrobials of human and veterinary importance in poultry sector of India,” J Food Saf, Vol. 40, pp. e12742, Dec 2019.
Google Scholar
12
-
Q. Chang, W. Wang, G. Regev-Yochay, M. Lipsitch, W. P. Hanage, “Antibiotics in agriculture and the risk to human health: how worried should we be?” Evol Appl., Vol. 8, pp.240-247, Mar 2015.
Google Scholar
13
-
Y. B. Kim, M. Y. Yoon, J. S. Ha, K. W. Seo, E. B. Noh, et al., “Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis,” Poult Sci., Vol. 99, pp. 1088-1095, Feb 2020.
Google Scholar
14
-
M. T. Rahman, M. A. Sobur, M. S. Islam, S. Ievy, M. J. Hossain, et al., “Zoonotic Diseases: Etiology, Impact, and Control,” Microorganisms, Vol. 8, pp. 1405, Sep 2020.
Google Scholar
15
-
O. Tenaillon, D. Skurnik, B. Picard, E. Denamur, “The population genetics of commensal Escherichia coli,” Nat Rev Microbiol., Vol. 8, pp. 207-217, Mar 2010.
Google Scholar
16
-
M. Mellata, “Human and Avian Extraintestinal Pathogenic Escherichia coli: Infections, Zoonotic Risks, and Antibiotic Resistance Trends,” Foodborne Pathogens and Disease, Vol.11, pp. 916-932, Oct 2013.
Google Scholar
17
-
G. Abdukhalilova, L. Kaftyreva, J. A. Wagenaar, B. Tangyarikov, A. Bektimirov, I. Akhmedov, et al., “Occurrence and antimicrobial resistance of Salmonella and Campylobacter in humans and broiler chicken in Uzbekistan,” Public Health Panorama, Vol. 2, pp. 340-347, 2016.
Google Scholar
18
-
C. Varga, M.T. Guerin, M .L. Brash, et al., “Antimicrobial resistance in fecal Escherichia coli and Salmonella enterica isolates: a two-year prospective study of small poultry flocks in Ontario, Canada,” BMC Vet Res, Vol. 15, pp. 464, Dec. 2019.
Google Scholar
19
-
I. S. Shanta, M. A. Hasnat, N. Zeidner, E. S. Gurley, E. Azziz‐Baumgartner, et al., “Raising backyard poultry in rural Bangladesh: financial and nutritional benefits, but persistent risky practices,” Transboundary and Emerging Diseases, Vol. 64, pp. 1454-1464, June 2017.
Google Scholar
20
-
D. V. T. Nair, K. Venkitanarayanan, A. Kollanoor Johny, “Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control,” Foods, Vol. 7, pp. 167, June 2018.
Google Scholar
21
-
H. Al-Talib, H. Hasan, C. Y. Yean, S. M. Al-Ashwal, M. Ravichandran, “Fatal necrotizing pneumonia caused by Panton-Valentine leukocidin-producing hospital-acquired Staphylococcus aureus: a case report,” Jpn J Infect Dis., Vol, 64, pp. 58-60, 2011.
Google Scholar
22
-
S. A. Mamza, G. O. Egwu, and G. D. Mshelia, “Beta-lactamase Escherichia coli and Staphylococcus aureus isolated from chickens in Nigeria,” Veterinary Italian Journal, Vol. 46, pp. 155-165, 2010.
Google Scholar
23
-
P. D. Stapleton, and P. W. Taylor, “Methicillin resistance in Staphylococcus aureus,” Science Progress, Vol. 85, pp. 57-72, Feb 2002.
Google Scholar
24
-
M. Cheesbrough, “District Laboratory Practice in Tropical Countries (2nd Edition),” English Language Book Society, London 2006, pp. 100-194.
Google Scholar
25
-
I. A. Merchant and R. A. Packer, “Veterinary Bacteriology and Virology,” 7th edn. TheIowa University Press, Ames, Iowa, USA, 1967. pp. 286-306.
Google Scholar
26
-
W. Bauer, W. M. M. Kirby, J. Sheris and M. Truck, “Antibiotic susceptibility testing by a standardized single disk method,” American J. Clin. Phath., Vol. 145, pp. 225-230, 1966.
Google Scholar
27
-
E. Pyzik, A. Marek, “Plasmid profile analysis and evaluation of antibiotic susceptibility of Staphylococcus aureus strains isolated from table chicken eggs,” Pol J Vet Sci., Vol. 16, pp. 307-312, 2013.
Google Scholar
28
-
A. Khan, R. Rind, M. Shoaib, A. A. Kamboh, G. A. Mughal, “Isolation, identification and antibiogram of Escherichia coli from table eggs,” J. Anim. Health Prod., Vol. 4, pp. 1-5, Dec 2016.
Google Scholar
29
-
A. Parveen, M. M. Rahman, M. Fakhruzzaman, M. Akter, and M. S. Islam, “Characterization of bacterial pathogens from egg shell, egg yolk, feed and air samples of poultry houses,” Asian Journal of Medical and Biological Research, Vol. 3, pp. 168-174, Aug 2017.
Google Scholar
30
-
S. Chaemsanit, A. Akbar and A. K. Anal, “Isolation of total aerobic and pathogenic bacteria from table eggs and its contents,” Food Appl. Biosci. J., Vol. 3, pp. 1-9. April 2015.
Google Scholar
31
-
M. Salihu, B. Garba and Y. Isah, “Evaluation of microbial contents of table eggs at retail outlets in Sokoto metropolis, Nigeria,” Sokoto J. Vet. Sci, Vol. 13, pp. 22-28, 2015.
Google Scholar
32
-
B. M. Hang’Ombe, R. N. Sharma, L. M. Tuchili and O. Skjerve, “Isolation of bacteria from table eggs in Zambia,” Indian J. A. S., Vol. 69, pp. 445-447, 1999.
Google Scholar
33
-
USDA (United State Department of Agriculture, Food Safety & Inspection Services), 2011. Shell eggs from farm to table. www.fsis.usda.gov, retrieved 12-04-2014.
Google Scholar
34
-
J. Bruce and E. M. Drysdale, Trans-shell transmission, “Microbiology of the avian egg,” Chapman and Hall, London. pp. 63-91, 1994.
Google Scholar
35
-
C. Papadopoulou, D. Dimitriou, S. Levidiotou, H. Gessouli, A. Panagiou, S. Golegou and G. Antoniades, “Bacterial strains isolated from eggs and their resistance to currently used antibiotics: Is there a health hazard for consumers?” Comp. Immunol. Microbiol. Infect. Dis., Vol. 20, pp. 35-40, 1997.
Google Scholar
36
-
K. R. Jain and R. Yadav, “Study of antibiotic resistance in bacteria isolated from table egg,” Int. J. Pharm. Bio. Sci., Vol. 8, pp. 668-674, Jan 2017.
Google Scholar
37
-
S. Eid, S. A. Nasef and A. M. Erfan, “Multidrug resistant bacterial pathogens in eggs collected from backyard chickens,” Assiut Vet. Med. J., Vol. 61, pp. 87-103, Jan 2015.
Google Scholar
38
-
A. Adesiyun, N. Offiah, N. Seepersadsingh, S. Rodrigo, V. Lashley and L Musai, “Antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from table eggs,” Food Cont., Vol. 18, pp. 306-311, 2007.
Google Scholar
39
-
A. Kilonzo-Nthenge, S. N. Nahashon, F. Chen and N. Adefope, “Prevalence and antimicrobial resistance of pathogenic bacteria in chicken and guinea fowl,” Poult. Sci., Vol. 87, pp. 1841-1848, 2008.
Google Scholar
40
-
I. G. Wilson, “Antimicrobial resistance of salmonella in raw retail chickens imported chicken portions, and human clinical specimens,” J. Food Prot., Vol. 67, pp.1220-1225, 2004.
Google Scholar
41
-
C. M. Schroeder, D. G. White and J. Meng, “Retail meat and poultry as a reservoir of antimicrobial-resistant Escherichia coli,” Food Microbiol., Vol. 21, pp. 249-255, 2004.
Google Scholar
42
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