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Pleurotus ostreatus mushroom was cultivated on sawdust supplemented with different substrates, Cassava peel, Yam peel, and dried plantain leaf. Various treatment combinations of control, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, 15.0% and 17.5% carried out. Preparation of spawn, inoculation, incubation, and harvesting were done. Growth parameters of mean height, weight, diameter of fruiting bodies, mean stem girth and number of fruiting bodies were evaluated. Results showed that sawdust supplemented at 10% treatment level with Cassava peel was significantly higher than substrates in weight (229.34 g), height (95.01 cm) and number of fruiting bodies (103.21).

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