Research Article

The Quality of Cryopreserved Dairy Bulls Semen along Supply-Chain Used for Artificial Insemination in Tanzania

Patricia P. Ngoda
National Artificial Insemination Centre, Tanzania
* Corresponding author
Isaac P. Kashoma
Department of Veterinary Surgery and Theriogenology, Sokoine University of Agriculture, Tanzania
George G. Msalya
Department of Animal, Aquaculture, and Range Sciences, Sokoine University of Agriculture (SUA), Tanzania
Athumani S. Nguluma
Department of Animal, Aquaculture, and Range Sciences, Sokoine University of Agriculture (SUA), Tanzania
DOI icon 10.24018/ejfood.2023.5.5.714

Read Counter
541

Downloads
300

Citations

Share

Article Sidebar

Submitted 2023-07-14
Published 2023-10-11

Read counter = 541 times

Table of Contents

Article Main Content

Several factors are said to influence semen quality in breeding bulls. This research was carried out to investigate the effect of season and cold value chains on semen quality parameters. A total of 1652 semen production records from 2018 to 2021 from 10 dairy bulls preserved at the National Artificial Insemination Centre (NAIC), USA River, Arusha, Tanzania, and 540 cryopreserved semen examined were analyzed using Analysis of Variance (ANOVA) and the results were expressed as mean ± standard error of the mean using statistical analysis system (SAS, 2004), to determine whether there were significant differences among variables tested. All semen traits (VOL, SPC, PM, TM, PM, TM, VER, MP, and HP) were significantly (P <0.05) affected by season and cold value chain. During the cold season, the highest values of SPC, PM, TM, PM, TM, VER, MP, and HP were observed as compared to that of the warm season. Furthermore, semen quality decreased gradually from pre-state (before freezing) to post-state (after freezing for 48 hours) with minor affection for semen motility. There was a higher decrease (P <0.05) in quality from cryo-stored semen straw to those of the same batch retrieved back from the field. The semen quality parameters were satisfactory in the first three stages when compared to the semen straw from the field which was observed to have poor motility which could be due to poor handling, leakage of storage and transportation containers, and sometimes the delay of refilling the LN2. Finally, during the cold season and in all stages of semen production the semen is qualitatively and quantitatively good.
Keywords: cold value chain season Semen quality

References

  1. Morrow CJ, Asher GW., Fisher, MW, Fennessy PF, Mackintosh CG. and Jabbour HN. Oestrous synchronization, semen collection and artificial insemination of farmed red deer (Cervus elaphus) and fallow deer (Dama dama). Animal Reproduction Science. 1993; 33(1–4):241–65.
     Google Scholar
  2. Webb DW. Artificial Insemination in Cattle. University of Florida, Gainesville. FIFAS Extension, DS. 2003: 1–4.
     Google Scholar
  3. Fuerst-Waltl B, Schwarzenbacher H, Perner C, Sölkner J. Effects of age and environmental factors on semen production and semen quality of Austrian Simmental bulls. Animal Reproduction Science. 2006; 95(1–2):27–37.
     Google Scholar
  4. Christensen P, Labouriau R, Birck A, Boe-Hansen GB, Pedersen J, Borchersen S. Relationship among seminal quality measures and field fertility of young dairy bulls using low-dose inseminations. Journal of Dairy Science. 2011; 94(4):1744–54.
     Google Scholar
  5. Ahmed N, Kathiresan D, Ahmed FA, Lalrintluanga K, Mayengbam P, Gali JM. Pattern of induced estrus and conception rate following Ovsynch and Ovsynch based gonadotropin-releasing hormone treatments initiated on day 6 of estrous cycle in repeat breeding crossbred cows. Veterinary World. 2016; 9(4):342–5.
     Google Scholar
  6. Pegg DE. Principles of cryopreservation. Methods in molecular biology 1257. Methods in Molecular Biology. 2015; 1257:3–19.
     Google Scholar
  7. Bailey JL, Bilodeau JF, Cormier N. Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. Journal of Androl. 2000; 21(1):1–7.
     Google Scholar
  8. Woods E, Thirumala S, Han X, Critser JK. Fundamental cryobiology of reproductive cells and tissues: Concepts and misconceptions. In: Donnez J, Kim SS, editors. Principles and Practice of Fertility Preservation. Cambridge: Cambridge University Press; 2011. p. 129–44.
     Google Scholar
  9. Lessard C, Parent S, Leclerc P, Bailey JL, Sullivan R. Cryopreservation alters the levels of the bull sperm surface protein P25b. Journal of Androl. 2000; 21(5):700–7.
     Google Scholar
  10. Nagata MB, Egashira J, Katafuchi N, Endo K, Ogata K, Yamanaka K, et al. Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility. Journal of Animal Science and Biotechnology. 2019; 10(1):91.
     Google Scholar
  11. Chaverio A, Machado L, Frijters A, Engel B, Woelders H. Improvement of parameters of freezing protocol for bull sperm using two osmotic supports. Theriogenol. 2006; 65:1875–90.
     Google Scholar
  12. Cooter PZ, Goolsby HA, Prien SD. Preliminary evaluation of a unique freezing technology for bovine spermatozoa cryopreservation. Reprod. Dom. Animal. 2005; 40:98–99.
     Google Scholar
  13. Chantler E, Abraham-Peskir JV. Significance of midpiece vesicles and functional integrity of the membranes of human spermatozoa after osmotic stress. Andrologia. 2004; 36(2):87–93.
     Google Scholar
  14. Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm free ability in pigs. Theriogenology. 2016; 85:47–64.
     Google Scholar
  15. Rossitto M, Marchive C, Pruvost A, Sellem E, Ghettas A, Badiou S, et al. Intergenerational effects on mouse sperm quality after in utero exposure to acetaminophen and ibuprofen. Faseb Journal. 2019; 33(1):339–57.
     Google Scholar
  16. Amann RP, Seidel GE Jr, Mortimer RG. Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet. Theriogenology. 2000; 54(9):1499–515.
     Google Scholar
  17. Bucak MN, Ataman MB, Baspinar N, Uysal O, Taspinar M, Bilgili A, et al. Lycopene and resveratrol improve post thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia. 2015; 47(5):545–52.
     Google Scholar
  18. Partyka A, Łukaszewicz E, Niżański W and Twardoń J. Detection of lipid peroxidation in frozen-thawed avian spermatozoa using C11-BODIPY581/591. Theriogenology. 2011; 75(9):1623–1629.
     Google Scholar
  19. Castro LS, Siqueira AFP, Hamilton TRS, Mendes CM, Visintin JA, Assumpção MEOA. Effect of bovine sperm chromatin integrity evaluated using three different methods on in vitro fertility. Theriogenology. 2018; 107:142–148.
     Google Scholar
  20. Susilawati T. Spermatology. 1st ed. University Brawijaya Press: Indonesia; 2011.
     Google Scholar
  21. Rurangwa E, Kime DE, Ollevier F, Nash JP. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture. 2004; 234(1–4):1–28.
     Google Scholar
  22. Amann RP, Waberski D. Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology. 2014; 81(1):5-17.
     Google Scholar
  23. Ansari MS, Rakha BA, Akhter S and Ashiq M OPTIXcell improves the postthaw quality and fertility of buffalo bull sperm. Theriogenology. 2016; 85:528-532.
     Google Scholar
  24. Bahmid NA, Karja NWK, Arifiantini RI. The quality of frozen friesian holstein semen after long-term storage. Tropical Animal Science Journal. 2023; 46.
     Google Scholar
  25. Arifiantini RI. Semen Collection and Evaluation Techniques in Animals. Bogor: IPB Press; 2012.
     Google Scholar
  26. SAS User’s Guide: Statistics. SAS (Statistical Analysis Systems). 2004.
     Google Scholar
  27. Kathiravan P, Kalatharan J, John J, Edwin M, Veerapandian C. Computer automated motion analysis of cros bred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes. Animal Reproduction Science. 2008; 105:9–17.
     Google Scholar
  28. Fréour T, Jean M, Mirallie S, Barriere P. Computer-assisted sperm analysis parameters in young fertile sperm donors and relationship with age. System Biology Reproduction Medicine. 2012; 58(2):102–6.
     Google Scholar
  29. Kathiravan PJ, Kalatharan G, Karthikeya K, Rengarajan H, Kadirvel G. Objective sperm motion analysis to assess dairy bull fertility using computer-aided system: A review Reproduction Dom Animal 46. A review Reproduction Dom Animal. 2011; 46:165–72.
     Google Scholar
  30. Nagy JJ, Topper EK, Gadella BM. A triple-stain flow cytometric method to assess plasmaand acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biology of Reproduction. 2003; 68:1828–35.
     Google Scholar
  31. Januskauskas A, Johannisson A, Rodriguez-Martinez H. Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology. 2003;6 0(4):743–58.
     Google Scholar
  32. Erdemli S, Isik E, Oztuna A, Karahuseyinoglu DS. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of Assisted Reproduction Genetic. 2008; 25(8):403–11.
     Google Scholar
  33. Silva PFN, Gadella BM. Detection of damage in mammalian sperm cells. Theriogenology. 2006; 65(5):958–78.
     Google Scholar
  34. Connell O, Mcclure M, Lewis N. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reproduction. 2002; 17(3):704–9.
     Google Scholar
  35. Aisah S, Isnaini N, Wahyuningsih S. Kualitas semen segar dan recovery rate sapi bali pada musim yang berbeda. [Quality of fresh semen and recovery rate of Bali cattle in different seasons]. Jurnal Ilmu-Ilmu Peternakan. 2017; 27(1):63-79. Indonesian.
     Google Scholar
  36. Malik A, Laily M, Zakir MI. Effects of long-term storage of semen in liquid nitrogen on the viability, motility and abnormality of frozen thawed Frisian Holstein bull spermatozoa. Asian Pacific Journal of Reproduction. 2015;4(1):22–5.
     Google Scholar
  37. Mammoto A, Masumoto N, Tahara M, Ikebuchi Y, Ohmichi M, Tasaka K, et al. Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biology of Reproduction. 1996; 55(5):1063–8.
     Google Scholar
  38. Mazur P, Koshimoto C. Is intracellular ice formation the cause of death of mouse sperm frozen at high cooling rates. Biology Reproduction. 2002; 66:1485–90.
     Google Scholar
  39. Fraser LJ, Strzezek W. Kordan. Post-thaw sperm characteristics following long-term storageof boar semen in liquid nitrogen. Animal Reproduction Science. 2014; 147:119–27.
     Google Scholar
  40. Curtis SE. Environmental management in animal agriculture. Iowa State University Press; 1983.
     Google Scholar
  41. Vilakazi DM. Factors affecting the quality of semen of Artificial Insemination dairy bulls in South Africa. A Magister Instituninos Agrariae (Animal Production). Master Thesis. University of Pretoria: 2003.
     Google Scholar
  42. Mandal DK, Kumar M, Tyagi S. Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls. Animal. 2010; 4(4):595–603.
     Google Scholar
  43. Koivisto MB, Costa MT, Perri SH, Vicente WR. The effect of season on semen characteristics and freezability in Bos indicus and Bos taurus bulls in the South Eastern region of Brazil. Reproduction Domestic Animal. 2009; 10:1439–531.
     Google Scholar
  44. Bhakat M, Mohanty TK, Raina VS, Gupta AK, Khan HM, Mahapatra RK, et al. Effect of age and season on semen quality parameters in Sahiwal bulls. Tropical Animal Health Production. 2011; 43(6):1161–8.
     Google Scholar
  45. Mostari MP, Hasanat MS, Azmal SA, Monira KN, Khatun H. Effect of seasonal variation on semen quality and herd fertility. Pakistan Journal of Biological Sciences. 2005; 8(4):581–585.
     Google Scholar
  46. Mathevon M, Buhr MM, Dekkers JCM. Environmental, management, and genetic factors affecting semen production in Holstein bulls. Journal of Dairy Science. 1998; 81(12):3321–30.
     Google Scholar
  47. Vilakazi DM, Webb EC. Effect of age and season on sperm morphology of Friesland bulls at an artificial insemination centre in South Africa. South African Journal of Animal Science. 2004; 34(1):62-69.
     Google Scholar