##plugins.themes.bootstrap3.article.main##

There is an increasing demand for functional food products developed from local resources. In this work, Lactobacillus (Lb.) rhamnosus yoba 2012 and Streptococcus thermophilus were used to produce a soy-soursop probiotic yogurt. Soy milk was supplemented with soursop pulp in amounts of 0%, 5%, 10%, and 15%. The mixtures were pasteurized at 85 °C for 15 s, cooled, inoculated with a starter and incubated at 45 °C for 24 h. Samples were taken at 0, 2, 4, 6 and 24 hours for analysis of pH, acidity, and Lb. rhamnosus yoba 2012 counts. Consumer acceptability and willingness to purchase were determined after 24 h of fermentation. The most acceptable sample was analyzed for nutritional composition. Lb. rhamnosus grew in the soy-soursop yogurt up to 8.1-9.3 log cfu/mL. The highest cell growth was observed in yogurt containing 5% soursop whereas the lowest was observed in yogurt containing 15% soursop. Yogurt pH reduced to 3.9-4.4, with 15% soursop having the lowest pH (p<0.05). Titratable acidity increased to 0.6%-0.9% lactic acid. All the yogurts were accepted although those containing 15% soursop had the lowest scores (5 = neither like nor dislike). There were no yeasts, molds or coliforms detected during the 21 days of cold storage (4 ℃) of all the yogurt. Therefore, adding soursop to soymilk produces a satisfactory probiotic soy-soursop yogurt.

References

  1. Lang F, Wen J, Wu Z, Pan D, Wang L. (2022). Evaluation of probiotic yoghurt by the mixed culture with Lactobacillus plantarum A3. Food Sci. Hum. Wellness. 2022 Mar 11(2):323–331. https://doi.org/10.1016/j.fshw.2021.11.006.
     Google Scholar
  2. Kerry RG, Patra JK, Gouda S, Park Y, Shin HS, Das G. Benefaction of probiotics for human health: A review. JFDA. 2018 Jul 1;26(3):927-39. https://doi.org/10.1016/j.jfda.2018.01.002.
     Google Scholar
  3. Drisko JA, Giles CK, Bischoff BJ. Probiotics in health maintenance and disease prevention. Altern Med Rev. 2003 May 1;8(2):143-55.
     Google Scholar
  4. Guandalini S. Probiotics for Diarrheal Diseases. Diarrhea: Diagnostic and Therapeutic Advances. 2011:459-74.
     Google Scholar
  5. Kort R, Sybesma W. Probiotics for everybody. Trends Biotechnol. 2012 Dec 1;30(12):613-615. https://doi:10.1016/j.tibtech.2012.09.002.
     Google Scholar
  6. Bisanz JE, Macklaim JM, Gloor GB, Reid G. Bacterial metatranscriptome analysis of a probiotic yogurt using an RNA-Seq approach. Int. Dairy J. 2014 Dec 1;39(2):284-292. https://doi.org/10.1016/j.idairyj.2014.07.010.
     Google Scholar
  7. Kort R, Westerik N, Mariela Serrano L, Douillard FP, Gottstein W, Mukisa IM, Tuijn CJ, Basten L, Hafkamp B, Meijer WC, Teusink B. A novel consortium of Lactobacillus rhamnosus and Streptococcus thermophilus for increased access to functional fermented foods. Microb. Cell Factories. 2015 Dec;14(1):1-4.https://doi.org/10.1186/s12934-015-0370-x.
     Google Scholar
  8. Mpofu A, Linnemann AR, Sybesma W, Kort R, Nout MJ, Smid EJ. Development of a locally sustainable functional food based on mutandabota, a traditional food in southern Africa. JDS. 2014 May 1;97(5):2591-9. https://doi:10.3168/jds.2013-7593.
     Google Scholar
  9. Wacoo AP, Mukisa IM, Meeme R, Byakika S, Wendiro D, Sybesma W, Kort R. Probiotic enrichment and reduction of aflatoxins in a traditional African maize-based fermented food. Nutrients. 2019 Jan 25;11(2):265. https://doi.org/10.3390/nu11020265.
     Google Scholar
  10. Shilpa V, Subrota H, Deepika Y. Biofunctionality of probiotic soy yoghurt. FNS. 2011 Jul 5;201. DOI:10.4236/fns.2011.25073.
     Google Scholar
  11. Kulkarni DS, Kapanoor SS, Girigouda K, Kote NV, Mulimani VH. Reduction of flatus‐inducing factors in soymilk by immobilized α‐galactosidase. Biotechnol. Appl. Biochem. 2006 Sep;45(2):51-7. https://doi.org/10.1042/BA20060027.
     Google Scholar
  12. Muthyala RS, Ju YH, Sheng S, Williams LD, Doerge DR, Katzenellenbogen BS, Helferich WG, Katzenellenbogen JA. Equol, a natural estrogenic metabolite from soy isoflavones: convenient preparation and resolution of R-and S-equols and their differing binding and biological activity through estrogen receptors alpha and beta. Bioorg. Med. Chem. 2004 Mar 15;12(6):1559-67. https://doi.org/10.1016/j.bmc.2003.11.035.
     Google Scholar
  13. Garcia K, Sriwattana S, No HK, Corredor JA, Prinyawiwatkul W. Sensory optimization of a mayonnaise‐type spread made with rice bran oil and soy protein. J. Food Sci. 2009 Aug;74(6):S248-54.https://doi.org/10.1111/j.1750-3841.2009.01203.x.
     Google Scholar
  14. Morton JF. Fruits of Warm Climates. Echo Point Books and Media. 2013.
     Google Scholar
  15. Lutchmedial M, Ramlal R, Badrie N, Chang-Yen I. Nutritional and sensory quality of stirred soursop (Annona muricata L.) yoghurt. Int J Food Sci Nutr. 2004 Aug 1;55(5):407-14. https://doi.org/10.1080/09637480400002800.
     Google Scholar
  16. Afzaal M, Saeed F, Asghar A, Shah YA, Ikram A, Ateeq H, Hussain M, Ofoedu CE, Chacha JS. Nutritional and Therapeutic Potential of Soursop. J. Food Qual. 2022 Aug 30;2022.https://doi.org/10.1155/2022/8828358.
     Google Scholar
  17. Badrie N, Schauss AG. Soursop (Annona muricata L.): composition, nutritional value, medicinal uses, and toxicology. In Bioactive foods in promoting health. 2010 Jan 1 (pp. 621-643). https://doi.org/10.1016/B978-0-12-374628-3.00039-6.
     Google Scholar
  18. Mazumder MA, Begum AA. Soy milk as source of nutrient for malnourished population of developing country: A review. Int. J. Adv. Sci. Res. 2016;5(6):192-203.
     Google Scholar
  19. Kosterina V, Yakovleva A, Koniaeva V, Iakovchenko N. Development of formulation and technology of non-dairy soy-coconut yogurt. 2020. https://doi.org/10.15159/AR.20.107.
     Google Scholar
  20. Fisberg M, Machado R. History of yogurt and current patterns of consumption. Nutr. Rev. 2015 Aug 1;73(suppl_1):4-7. https://doi.org/10.1093/nutrit/nuv020.
     Google Scholar
  21. AOAC. Official Methods of Analysis (Volume 1). Washington DC: Association of Official Analytical Chemists; 1990.
     Google Scholar
  22. Microbiology of food and animal feeding stuffs—Horizontal method for the enumeration of coliforms—Colony-count technique. (n.d.). ISO Standard 4832:2006.
     Google Scholar
  23. Microbiology of food and animal feeding stuffs—Horizontal method for the enumeration of yeasts and moulds—Part 1: Colony count technique in products with water activity greater than 0,95. (n.d.)., ISO Standard 21527-1:2008.
     Google Scholar
  24. AOAC. Official Methods of Analysis, 16th Edition, 5th Revision, Washington DC: Association of Official Analytical Chemists; 1999.
     Google Scholar
  25. Jeon IK. Carbohydrates and sugars. In: Analyzing food for nutrition labeling and hazardous contaminants. Ed. Ike J. Jeon and William G. Ikins. New York: Marcel Dekker Inc; 1995.
     Google Scholar
  26. Gustaw W, Kordowska-Wiater M, Kozioł J. The influence of selected prebiotics on the growth of lactic acid bacteria for bio-yoghurt production. Acta Sci. Pol. Technol. Aliment. 2011 Dec 30;10(4):455-66.
     Google Scholar
  27. Yáñez R, Marques S, Gírio FM, Roseiro JC. The effect of acid stress on lactate production and growth kinetics in Lactobacillus rhamnosus cultures. Process Biochem. 2008 Apr 1;43(4):356-61. https://doi.org/10.1016/j.procbio.2007.12.014.
     Google Scholar
  28. Hayakawa K, Sansawa H, Nagamune T, Endo I. High density culture of Lactobacillus casei by a cross-flow culture method based on kinetic properties of the microorganism. J. Biosci. Bioeng. 1990 Jan 1;70(6):404-8.https://doi.org/10.1016/0922-338X(90)90122-D.
     Google Scholar
  29. Luckow T, Delahunty C. Which juice is ‘healthier’? A consumer study of probiotic non-dairy juice drinks. Food Qual Prefer. 2004 Oct 1;15(7-8):751-9. https://doi.org/10.1016/j.foodqual.2003.12.007.
     Google Scholar
  30. Tripathi MK. Giri sK. Probiotic functional foods: survival of probiotics during processing and storage. J. Funct. Foods. 2014;9:225-41.https://doi.org/10.1016/j.jff.2014.04.030.
     Google Scholar
  31. Mishra S, Mishra HN. Technological aspects of probiotic functional food development: a review. Nutrafoods. 2012 Dec;11:117-30. https://doi.org/10.1007/s13749-012-0055-6.
     Google Scholar
  32. Viander B, Mäki M, Palva A. Impact of low salt concentration, salt quality on natural large-scale sauerkraut fermentation. Food Microbiol. 2003 Aug 1;20(4):391-5.https://doi.org/10.1016/S0740-0020(02)00150-8.
     Google Scholar
  33. Mukisa IM, Birungi SW. Proximate composition, acceptability and stability of probiotic dairy yoghurt containing cooking banana/matooke puree and Lactobacillus rhamnosus yoba. J. Microbiol. Biotechnol. Food Sci. 2021, 343-347.
     Google Scholar
  34. Yoghurt — Specification. East African Community, EAS 33:2019.
     Google Scholar
  35. Kamber U, Harmankaya S. The effect of fruits to the characteristics of fruit yogurt. Pakistan Journal of Agricultural Sciences. 2019 Apr 1;56(2).
     Google Scholar
  36. Nyanzi R, Jooste PJ, Abu JO, Beukes EM. Consumer acceptability of a synbiotic version of the maize beverage mageu. Dev. South. Afr. 2010 Sep 1;27(3):447-63.
     Google Scholar
  37. Hasimah HA, Faridah MS, Hasanah MR. Chemical, microbiological and sensory quality changes of semi-cooked" roti canai" during low temperature storage. MARDI Res. J. 1991;19:129-35.
     Google Scholar
  38. Badrie N, Schauss AG. Soursop (Annona muricata L.): composition, nutritional value, medicinal uses, and toxicology. InBioactive foods in promoting health 2010 Jan 1 (pp. 621-643). Academic Press.
     Google Scholar
  39. Yogurt—Enumeration of characteristic microorganisms—Colony-count technique at 37 degrees C. ISO Standard 7889:2003.
     Google Scholar
  40. Karovičová J, Kohajdová Z, Šimko P, Lukáčová D. Using capillary isotachophoresis for the determination of biogenic amines and D‐isocitric acid in food products. Food/Nahrung. 2003 Jun 1;47(3):188-90. https://doi.org/10.1002/food.200390044.
     Google Scholar
  41. Heldman DR, Hartel RW. Refrigerated Storage. In: Principles of Food Processing. Food Science Texts Series. Boston, MA: Springer. 1999.
     Google Scholar
  42. Steinkraus KH. (1994). Nutritional significance of fermented foods. Food Res. Int. 1994 Jan 1;27(3):259-67. https://doi.org/10.1016/0963-9969(94)90094-9.
     Google Scholar