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Cashew (Anacardium occidentale L.) trees are threatened by my many fungal diseases, among which pestaliotiopsis occupies an important place in terms of damage created in Cameroon. The aim of the present study is to evaluate the effects of aqueous extract of neem seed (AENS) on the development of Pestalotia heterocornis, the causative agent of pestalotiopsis of cashew. Isolates of P. heterocornis were obtained from diseased cashew leaves collected in the orchads. The concentrations of AENS were C1 = 500 μg/ml, C2 = 50 μg/ml and C3 = 5 μg/ml. Mancozeb 80 WP (Mz) was used as positive control. Effect of Mancobeb 80WP on the growth and spore germination of isolates were performed. The effect of AENS on the plants was evaluated in vivo. Four (4) isolates of P. heterocornis were used. AENS C1 concentration reduced the growth of all isolates. The percentage of inhibition was ranged from 45 to 90 % with the other concentrations. AENS did not inhibited spore germination (0.0 %). But Mz totally inhibited conidia germination of all isolates. In vivo, AENS protected plants against P. heterocornis. AENS can be integrated in management of cashew plants against P. heterocornis.

References

  1. I. B. L. Coutinho, F. C. O. Freire, J. S. Lima, F. J. T. Gonçalves, A. R. Machado, A. M. S. Silva, and J. E. Cardoso, "Diversity of genus Lasiodiplodia associated with perennial tropical fruit plant in north eastern Brazil". Plant Pathol. Vol. 66, no 1, pp. 90–104, June 2016.
     Google Scholar
  2. F. Marlos, C. De lacerda, F.E. Gomes, B.C. De Abreu, T.J. Prisco, "Physiology of cashew plants grown under adverse conditions" Brazilian Journal of Plant Physiology, vol. 19, no. 4, pp. 449-461, 2007.
     Google Scholar
  3. E. Lacroix, "Les anacardiers, les noix de cajou et la filière anacarde à Bassila et au Bénin.Projet de Restauration des Ressources Forestières de Bassila, GTZ République du Bénin" pp. 1-75, 2003.
     Google Scholar
  4. E.K.A. Kouassi, "Contribution à la valorisation des sous-produits agricoles en bioproduits", Thèse de doctorat de l’Université de Toulouse, pp. 1-169, 2018.
     Google Scholar
  5. Y. Hamawa, A. Dona, O.N. Kanmegne, C. Mbaye, Niwah, J.M.D.K. Awono, P.M. Mapongmetsem, "Effet du poids de noix et de la dose d’engrais sur la germination et la croissance de l’anacardier (Anacardium occidentale L., Anacardiaceae) dans la savane guinéenne du Cameroun". Afrique Science, vol. 15, no. 5, pp. 302–312, 2019.
     Google Scholar
  6. E.S.C.A. Dedehou, J. Dossou, M.M. Soumanou, Etude diagnostique des technologies de transformation de la pomme de cajou en jus au Bénin. International Journal of Biological and Chemical Sciences, vol. 9, no. 1, pp. 371–387, February 2015.
     Google Scholar
  7. O.O. Adeigbe, F.O. Olasupo, B.D. Adewale, and A.A.Muyiwa, "A review of cashew research and production in Nigeria in the last four decades" Science Research Essays, vol. 10, pp. 196-209, 2015.
     Google Scholar
  8. F. M. P. Viana, J. E. Cardoso, and H. A. O. Suraiva, "First report of a bacterial leaf and fruit spot of cashew nut (Anacardium occidentale L.) caused by Xanthomonas campestris pv. Mangiferae indicae in Brazil". Plant Dis., vol. 91, no. 10, p. 1361, September 2007.
     Google Scholar
  9. L. C. A. Afouda, V. Zinsou, R. K. Balogoun, A. Onzo, and B.C. Ahohuendo, "Inventaire des agents pathogènes de l’anacardier (Anacardium occidentale L.) au Bénin". vol. 73, pp. 13-19 2013.
     Google Scholar
  10. E. O. Uwagboe, S. O. Adeogoun, and S. O. Odebode, "Constraints of farmers in cashewproduction: A case study of Orire L.G.A. of Oyo state, Nigeria". ARPN. Journal ofAgricultural and Biological Science, vol. 5, no. 4, pp. 27-31, 2010.
     Google Scholar
  11. N.V. Noiha, L. Zapfack, D. V. Awe, N. Witanou, B. Nyeck, J.D. Ngossomo, M.R. Hamadou, C.D. Chimi, R.B. Tabue-Mbobda, "Floristic structure and sequestration potential of cashew agroecosystems in Africa: A case study from Cameroon" Journal of Sustainable Forestry,vol. 36, no. 3, pp. 277-288, March 2017.
     Google Scholar
  12. J.P. Ngoh Dooh, D.B.C. Asta, B. Djile, T.D.B. Tchoupou, A. Heu, S.B. Mboussi, T.W.N. Kuate and Z. Ambang, "Major Fungi Diseases of Cashew Trees (Anacardium occidental L.) in Cameroon" Journal of Agricultural Science, vol. 13, no. 3, pp. 124-134, February 2021. DOI:10.5539/jas.v13n3p124.
     Google Scholar
  13. M. Mazarotto, M.P. Bovolini, M.F.B. Muniz, H.R. Ricardo, L.R.S. Reiniger, F. Álvaro, A.F.S. Dos Santos, "Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil". Brasília, vol. 49, pp. 440-448, 2014.
     Google Scholar
  14. N. Silué, S. Soro, T. Koné, K. Abo, M. Koné, and D. Koné, "Contrôle chimique et biologique des nécroses foliaires de l’anacardier (Anacardium occidentale L.) en CI. Agronomie Africaine", vol. 30, no. 1, pp. 107-122, 2018.
     Google Scholar
  15. D. Tonon, R. Sikirou, A.C. Adomou, V. Zinsou, B. Zocli, K. N’Djolosse, and S. Bello, Efficacité des fongicides Mancozèbe 80 WP et Chlorothalonil-Carbendazime 65 SC contre Colletotrichum gloeosporioides agent causal de l’anthracnose de l’anacardier au Bénin. Int. J. Biol. Chem. Sci., vol. 11, no. 5, pp. 2093-2105, Novembre 2017, DOI: https://dx.doi.org/10.4314/ijbcs.v11i5.13.
     Google Scholar
  16. M. Ahmad, and M.I. Aril, "Resistance of beet armyworm Spodoptera exigua (Lepidoptera: noctuidae) to endosulfan, organophosphorus an pyroheoid insecticides in Pakistan. Crop prot, vol. 29, no. 12, pp. 1428-1433, 2020.
     Google Scholar
  17. J. Wang, J. Li, J. Cao, and W. Jiang, "Antifungal activities of neem (Azadirachta indica) seed kernel extracts on post harvest diseases in fruits. African Journal of Microbiology Research, vol. 4, no. 11, pp. 1100-1104, May 2010.
     Google Scholar
  18. R.C. Saxena, Insecticides from Neem. In J.T. Arnason, B.J.R.Philogene and P. Morand (eds) Insecticides of Plant Origin.American Chemical Society Symposium series, vol. 387, pp. 110 – 35, 1989.
     Google Scholar
  19. J. Pohe, et T. Agneroh, "L’huile des grains de neem, un fongicide alternatif de la cacaoyère" Journal of Applied Biosciences, vol. 62, pp. 4644–4652, February 2013.
     Google Scholar
  20. F. Mamadou, "Nouveau fractionnement de la graine de neem (Azadirachta indica Juss) sénégalais: production d’un biopesticide d’huile et de tourteau. Thèse en vue de l’obtention du Doctorat de l’Université de Toulouse, pp. 1-267, 2010.
     Google Scholar
  21. A.J. Gnago, M. Danho, T.A. Agneroh, I.K. Fofana, et A.G. Kohou, "Efficacité des extraits de neem (Azadirachta indica) et de papayer (Carica papaya) dans la lutte contre les insectes ravageurs du gombo (Abelmochus esculentus) et du chou (Brassica oleracea) en Côte d’Ivoire" International Journal of Biological and Chemical Sciences, vol. 4, no. 4, pp. 953-965, 2010.
     Google Scholar
  22. S.B. Mboussi, Z. Ambang, and J.P. Ngoh Dooh, "Aqueous extracts effects of seeds of Thevetia peruviana and Azadirachta indicaon the development of Phytophthora megakaryain locality of Biakoa (Cameroon)". Journal of Applied Biosciences, vol. 105, pp. 10042–10054, September 2016. DOI: 10.4314/jab.v105i1.1.
     Google Scholar
  23. O.M. Gupta, S.R. Kotasthane, and M.M. Khare, 1986. "Fusarium wilt of chickpea (Cicer arietinum)" Agricult. Rev. vol. 7, pp. 87-97.
     Google Scholar
  24. S. S. N. Maharachchikumbura, L.D. Guo, E. Chukeatirote, A. H. Bahkali, K. D. Hyde, Pestalotiopsis—morphology, phylogeny, biochemistry and diversity. Fungal Diversity, vol. 50, pp. 167–187, Aughust 2011 DOI 10.1007/s13225-011-0125-x.
     Google Scholar
  25. S.D. Joshi, R. Sanjay, U.I. Baby, A. Mandal, "Molecular characterization of " spp. associated with tea (Camellia sinensis) in southern India using RAPD and ISSR markers" Indian J. Biotechnol, vol. 8, no. 4, pp. 377–383, November 2009.
     Google Scholar
  26. H. L. Barnett, and B. B. Hunter, " Illustrated genera of imperfecti fungi." 4th edition, MacMilan, New York, USA, 1987.
     Google Scholar
  27. S.R.Singh, L.E.N. Jackai, G. Thottappilly, K.F. Cardewell, and G.O. Myers, "Status of research on constraints to cowpea production, In Biotechnology: enchancing research on tropical crops in Africa éd, pp. 21-26, 1993.
     Google Scholar
  28. S. Nyassé, C. Cilas, C. Hérail, and G., Blaha, "Leaf inoculation as an early screening test of cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease" Crop Protection, vol. 14, pp. 657-663, December 1995.
     Google Scholar
  29. N. Dohou, K. Yamni, A. Badoc, et A. Douira, "Activité antifongique d’extraits de Thymelaea lythroides sur trois champignons pathogènes du riz. Bull. Soc. Pharm. Bordeaux, vol. 143, pp. 31-38, 2004.
     Google Scholar
  30. M.S.H. Moustafa, A.M. Hala, El-Dakar, and M. A.A. Asmaa, "Pestalotia leaf Spot a New Disease affect Guava Trees in Egyp," International Journal of Scientific & Engineering Research, vol. 6, no. 10, pp 1306-1312, October 2015.
     Google Scholar
  31. L.M. Keith, M.E.Velasquez, and F.T. Zee, "Identification and Characterization of Pestalotiopsis spp. Causing Scab Disease of Guava, Psidium guajava, in Hawaii" Plant Disease vol. 90, no. 1, pp. 17-23, 2006. DOI: 10.1094/PD-90-0016.
     Google Scholar
  32. H.L. Hu, R. Jeewon, D.Q. Zhou, T.X. Zhou, and K.D. Hyde, "Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: evidence from rDNA and β-tubulin gene phylogenies. Fungal Divers, vol. 24, pp. 1-22, Janvier 2007.
     Google Scholar
  33. D.S.S. Shuping, and J.N.Eloff, "The use of plants to protect plants and food against fungal pathogens: a review" Afr J Tradit Complement Altern Med., vol. 14, no. 4, pp. 120-127, 2017.
     Google Scholar
  34. B. Shu, O. Wang, J. Hu, M. Huang, Hu. and G.A. Zhong, "comprehensive etudy on apoptosis induction by azadirachtine in spodoptera frugiperda cultured cell line sf9.Arch Insect Biochem physiol", Arch. Insect Biochem, vol. 89, no. 3, pp. 153–68, 2015.
     Google Scholar
  35. B. Camara, E. Dick, A. Sako, D. Kone, C. Kanko, M.-A.-D. Boye, S. Ake, and A. Anno, Lutte biologique contre Deightoniella torulosa (Syd.) Ellis, par l’application des huiles essentielles d’Eucalyptus platyphylla F. Muell. et de Melaleuca quinquenervia L. Phytothérapie et écologie, vol. 8, pp. 240–244. July 2010. https://doi.org/10.1007/s10298-010-0568-3.
     Google Scholar
  36. F. M. Kassi, O. J. Badou, Z. F. Tonzibo, Z. Salah, L. D. G. E. Amari, et D. Koné, "Action du fongicide naturel NECO contre la cercosporiose noire (Mycosphaerella fijiensis Morelet) chez le bananier plantain (AAB) en Côte d’Ivoire". J. Appl. Biosci. Vol. 75, pp. 6183-6191, 2014.
     Google Scholar
  37. FRAC, "Fungicide Resistance Action Comittee. Code List©*2020: Fungal control agents sorted by cross resistance pattern and mode of action" p.16, 2020.
     Google Scholar
  38. N. Silué, K. Abo, B. Camara, S. Soro, G.M. Ouattara, S. Tuo, M. Koné, and D. Koné, "Effect of some Synthetic Fungicides on the in vitro Growth of Colletotrichum gloeosporioides, Causative Agent of Cashew Tree Anthracnose in Côte d’Ivoire" Asian J. Crop Sci. vol. 9, pp.149-158, April 2017.
     Google Scholar
  39. O. P. N. Effa, P. F. Niemenak, M. L. Djocgoue, M. Ondobo, and N. D, "Omokolo, Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans". Afr. J. Biotechnol, vol. 2, pp. 672-268, September 2015.
     Google Scholar
  40. S. S. L. Surendra, S. Godara, A. Gangopadhayaya, and K.S. Jadon, "Induced resistance against Alternaria brassicae blight of mustard through plant extracts", Archives of Phytopathology and Plant Protection, vol. 45, no. 14, pp. 1705-1714, 2012.
     Google Scholar